Pestalotiopsis bacterial strain preservation method

一种拟盘多毛孢菌种保藏方法

Abstract

The invention discloses a pestalotiopsis bacterial strain preservation method. The method comprises the following steps: firstly, bacterial strains are inoculated in a middle position of a medium; secondly, when hyphae grow out, a disinfection filter paper sheet is put around the inoculation position; thirdly, after the hyphae cover the filter paper sheet, the filter paper sheet is taken off and is put in a disinfection preservation pipe; fourthly, the pipe opening of the preservation pipe is sealed, classification and marking are carried out, and storage is carried out at a temperature of -80 DEG C to 4 DEG C. The preservation method employs the disinfection qualitative filter paper sheet and the EP pipe for storage, and the problems of pollution of infectious microbes and mites and water evaporation in the medium are avoided. the bacterial strain activation survival rate is high, the storage time is long, pollution is not easy, operation is simple and convenient, material consumption is low, operation is short, consumed time is short and the occupied space is small.
本发明公开了一种拟盘多毛孢菌种保藏方法,包括以下步骤:(1)将菌种接种于培养基中间位置;(2)待菌丝长出时,将灭菌滤纸片摆放在接种位置周围;(3)待菌丝覆盖滤纸片之后取下滤纸片,放在灭菌的保存管中;(4)用密封保存管管口,分类标记,在温度为-80℃~4℃的条件下保存。本发明拟盘多毛孢菌株保存方法,采用灭菌定性滤纸片和EP管保存,避免了杂菌及螨类的污染,以及培养基内水分蒸发的问题,菌株的活化成活率高,保存时间长,不易污染,而且操作简便易行,耗材少,工序短,耗时少,占空间小等优点。

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